Features
*1:Optional
*2: Autosamplers with or without thermostat are available
*3: Pumps with or without Auto-purge valve are available
Module lineup
Pump
5110: Pressure up to 40 MPa
5160: Pressure up to 60 MPa
| Item | 5110 | 5160 |
|---|---|---|
| Operating flow late range | 0.001 to 9.999 mL/min | 0.001 to 5.000 mL/min |
| Maximum operating pressure | 40 MPa (0.001 to 5.000 mL/min) 20 MPa (5.001 to 9.999 mL/min) | 60 MPa (0.001 to 2.500 mL/min) 30 MPa (2.501 to 5.000 mL/min) |
| Dimensions | 340(W) × 440(D) × 140(H) mm | |
| Number of mixed solvents*1 | Up to 4 | |
*With low pressure gradient unit (optional)
Autosampler
5260: Withstand pressure 60 MPa, Loop injection method
5280: Withstand pressure 60 MPa, Direct injection method
| Item | 5260 | 5280 | |
|---|---|---|---|
| Sample injection system | Loop injection method (Cut injection, All volume injection, Full loop injection method) | Direct injection method | |
| Withstand pressure | 60 MPa | 60 MPa | |
| Washing function | Equipped with built-in washing pump Two-solvent washing function | Wash solvent delivery by a syringe | |
| Sample rack temperature control | 1 to 45 °C | 1 to 35 °C | |
| (Temperature setting range) | Capable of cooling and heating | Cooling only | |
| Sample capacity | Standard | 120 × 1.5 mL | 200 × 1.5 mL |
| Optional | 72 × 4 mL | 128 × 4 mL | |
Column Oven
5310: Horizontal type
6310: Vertical type
| Item | 5310 | 6310 |
|---|---|---|
| Temperature setting range | 1 to 85 °C (1 °C step) | 4 to 90 °C (1 °C step) |
| Temperature control range | [Ambient temperature -15 °C] to [Ambient temperature +60 °C] and range of the temperature setting | [Ambient temperature -15 °C] to [Ambient temperature +75 °C] and within temperature setting range |
| Column capacity | 300 mm × 3 (Maximum) | |
| Built-in 3-liter water tank | - | Available |
| Dimensions | 410(W) × 440(D) × 140(H) mm | 165(W) × 515(D) × 689(H) mm (Legs are not included) |
Detector
5410: UV Detector
5420: UV-Vis Detector
5430: Diode Array Detector
5440: Fluorescence Detector
5450: RI Detector
5610: MS Detector
| Item | 5410 UV Detector | 5420 UV-Vis Detector | 5430 Diode Array Detector |
|---|---|---|---|
| Optical system | Double-beam ratio photometric system | Single-beam ratio photometric system | |
| Wavelength range | 190 to 600 nm | 190 to 900 nm | 190 to 900 nm |
| Flow cell | Optical path length 10 mm | ||
| Simultaneous measurement | Two-wavelength* | spectrum acquisition | |
| Dimensions | 340(W) × 440(D) × 140(H) mm | ||
| Item | 5440 Fluorescence Detector |
|---|---|
| Light source | Xe lamp, Hg lamp for checking wavelength |
| Wavelength range | Ex: 200 to 850 nm Em: 250 to 900 nm (Change photomultiplier at 731 nm or more) |
| Flow cell | Irradiation volume 12 µL |
| Dimensions | 340 (W) × 440 (D) × 280 (H) mm |
| Item | 5450 RI Detector |
|---|---|
| Refractive index range | 1 to 1.75 |
| Temperature control range | OFF, and 30 to 50 °C |
| Dimensions | 340 (W) × 440 (D) × 140 (H) mm, excluding projections |
| Item | 5610 MS Detector |
|---|---|
| Measurement mass range (m/z) | 20 to 1,000 |
| Ion source | Electrospray ionization (ESI) |
| Dimensions | 440 (W) × 610(D) × 430 (H) mm |
| N2 gas usage | Max flow rate 3.4 L/min, Pressure 300 ± 20 kPa |
Organizer
| Item | Organizer |
|---|---|
| Output power | DC24 V, 450 W Supplies power to one pump, one autosampler, one detector (UV detector, UV-Vis detector, Diode array detector, or RI detector), and one interface control board |
| Bottle capacity and space | 1.0 L bottle × 6 and 500 mL bottle × 3(Maximum), 314(W) × 280.8(D) mm |
| Dimensions | 340 (W) × 420 (D) × 200 (H) mm |
Data
System Performance
Analysis Example from the Chromaster 60 MPa System
The 60 MPa system, which can be used with columns containing 2.0 µm or smaller particles, and core-shell columns, is capable of conventional HPLC analysis and also ensures improved resolution performance and shorter analysis time.
| Sample | Alkylphenones |
|---|---|
| Column | LaChrom II C18 (5 µm) 4.6 mm I.D. × 150mmL LaChromUltra II C18 (1.9 µm) 2.0 mm I.D. × 50 mmL |
| Column temperature | 40 °C |
| Mobile phase | A: H2O, B: CH3CN |
| Gradient mode | High Frequency Mode |
| Wavelength | 247 nm |
The following options are used with UHPLC column:
- Low dispersion piping kit
- Semi-micro mixer (200 µL)
- Semi-micro flow cell unit
Example of Carry-over analysis (5260)
Even when highly adsorbing Chlorhexidine is analyzed, the carry-over is extremely low.
Easy to use
Analysis Example from the Chromaster 60 MPa System
Most optional accessories are internally mounted to reduce the HPLC system's height. The handle located on the front side of the organizer moves vertically for easy access to solvent bottles.
Module operations and the replacement of consumable and maintenance parts can be performed from the front side.
With attention to detail on the housing of tubes and wires, the system keeps tubes from getting tangled up, ensures the ease of replacement, and provides adequate seismic stability.
Column management system (optional)
Hitachi's column management system can manage the Log information on analytical columns and guard columns from any manufacturer.
Log information can be written and read through a connector mounted on the column oven or USB port in the computer. ID Tags can be used repeatedly*.
*Approximate read/write life time : 100,000 times
Wakeup (automatic pre-analysis tasks) and Sleep (automatic post-analysis tasks) programs
Automatic system Wakeup and Sleep from GUI
- In Conditioning, up to 10 programs can be created by combining any of the module settings, such as pre-analysis tasks of system (Wakeup), and post-analysis tasks of system (Sleep).
- For Wakeup program ending time, you can specify any time on current day, the following day, or two days later.
- The Sleep program starts at a specified time on the current day/the following day, or after the end of a continuous analysis run.
Automated system standby can reduce the amount of time required to make preparations for an analysis run.
Applications
Here are two examples of experiments that can be done using the Chromaster system:
- Column switching valves enable automation of the pretreatment process such as biological sample enrichment, removing impurities and other processes.
- The ability to switch between two different wavelengths in real time can be used to resolve the co-elution of impurities in drug products.
Pharmaceutical
Application for Biomedicines - Peptide Mapping Method -
Analysis of Two New Drugs in the Japanese Pharmacopoeia Sixteenth Edition
Analysis Example for Drugs in the Japanese Pharmacopoeia Sixteenth Edition (Acetylcysteine)
Automation of Pretreatment by Column-Switching
Analysis of decomposition products in aspirin by application of two-wavelength detection
Analysis of anti-mycoticagents
Simultaneous analysis of chlorzoxazoneand its metabolites
Simultaneous Analysis of Testosterone and Metabolites
Simultaneous Analysis of Phenoxyethanol and Parabens
Simultaneous Analysis of Main and Trace Components by High-sensitivity DAD
Analysis of Combination Drugs (Identification and Quantitative Analysis by DAD)
Food
Analysis of Bis(2-ethylhexyl) phthalate(DEHP) in Drinking Water
Analysis Example of Aflatoxin M1
Highly Sensitive Analysis of Organic Acids
Simultaneous Analysis of Fat-soluble Vitamins
Simultaneous Analysis of Water-Soluble Vitamins
Environment
Simultaneous analysis of four agricultural chemical components
Analysis of 2,4-DNPH-Derivatized Aldehydes
Simultaneous Analysis of Main and Trace Components by High-sensitivity DAD
NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.